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      Peptidomics means the comprehensive qualitative and quantitative analysis of peptides and small proteins. It is increasingly used in biomarker discovery and achieves promising results. Up to now the pathophysiology of major depressive disorder MDD is still partially elucidated. Moreover, the diagnosis of MDD is mainly based on a cluster of clinical symptoms. Therefore we aim to discover diagnostic markers for MDD and perform further investigation in the pathological mechanism of this mental disorder. Recent years, we collected various kinds of bio-samples including brain tissue, cerebrospinal fluid, blood and urine from human, macaca monkeys and rat/rice. Meanwhile a number of studies of MDD have been performed with MALDI-TOF MS and LC-MS/MS and obtain a preliminary result.